Refugia By Rewild Genomics

Your VCF file,
turned into a
plain-English
report

Conservation organizations collect field samples, send them to a sequencer, and get back genomic data they can't interpret. This platform closes that gap — population genetics analysis with no command line, no bioinformatician required.

7
Analysis types
< 10m
Typical run time
0
Command line steps
app.refugia.bio
◉ Dashboard
↑ New analysis
≡ Jobs
◈ Settings
Dashboard
Analyses
4
Species
3
Reports
2
Gray Wolf · JOB-004 Running
Pronghorn · JOB-003 Complete
Bison · JOB-001 Complete
Sage-Grouse · JOB-002 Failed
VCF Upload LD Pruning · PLINK2 Principal Component Analysis ADMIXTURE K=2–8 Pairwise FST · Weir-Cockerham Heterozygosity · FIS ROH Detection FROH Inbreeding Coefficients PDF Report Generation Population Structure Allelic Richness VCF Upload LD Pruning · PLINK2 Principal Component Analysis ADMIXTURE K=2–8 Pairwise FST · Weir-Cockerham Heterozygosity · FIS ROH Detection FROH Inbreeding Coefficients PDF Report Generation
The problem

Field data that goes
nowhere

Most conservation organizations can collect samples. Most sequencing cores can return a VCF. Almost none have the bioinformatics staff to do anything with it.

01
Samples collected in the field

Blood, tissue, or scat samples from camera trap sites, wildlife crossings, or reintroduction monitoring.

This part works fine
02
Sequencing returns a VCF file

A variant call format file lands in the organization's inbox. 50,000–500,000 lines of genotype data for every sample.

This part works fine
03
The VCF sits unopened

Without PLINK, R, Python, and a population genetics background, the file is unreadable. Most small orgs don't have any of those.

This is where things break
04
Decisions get made without the data

Reintroduction site selection, source population pairing, inbreeding risk — all decided on intuition rather than genetic evidence.

Conservation outcomes suffer
How it works

Upload once.
Understand everything.

01
Upload your VCF

Drag-and-drop any standard VCF or VCF.gz file from any sequencing platform. The platform validates format, checks for biallelic SNPs, and confirms sample IDs in under a minute.

VCF · VCF.gz Any sequencer Validation
02
Label your populations

Assign sample IDs to geographic populations using the visual mapping interface. Bulk-select by region, apply saved labels from previous runs, or let the platform suggest groupings from the data.

Population labels Saved templates Bulk assign
03
Configure and run

Choose which analyses to run — PCA, ADMIXTURE, FST, ROH — and set parameters like MAF threshold and LD window. Sensible defaults are pre-filled for wildlife conservation use cases.

PLINK2 ADMIXTURE FST · ROH
04
Download the report

A PDF report written in plain English, with figures your board can read and statistics your agency partners can cite. Interpretation is included — not just the numbers.

PDF export Plain English Citable results
Analysis outputs

What you get
in the report

Seven standard analyses run automatically on every uploaded VCF. Each includes a figure, the underlying statistics, and a plain-language interpretation of what the numbers mean for conservation management.

Population structure

Principal Component Analysis

Scatter plot of samples in PC space, colored by population assignment. Identifies clusters, outliers, and admixed individuals. Variance explained reported for each PC.

PC1 (18.3%) PC2 (9.1%)
Ancestry

ADMIXTURE Structure

Stacked bar chart showing ancestry proportions per individual across K=2 to K=8. Cross-validation selects the best K automatically. Admixed individuals are flagged for review.

Pop A Pop B Pop C Admixed K=3
Differentiation

Pairwise FST Heatmap

Weir-Cockerham pairwise FST values across all population pairs, visualized as a heatmap. Populations with high FST are genetically isolated — important for translocation planning.

Inbreeding

ROH and FROH Coefficients

Runs of homozygosity detected per individual using PLINK2. FROH reported per sample, with population-level inbreeding summaries and flagging of high-risk individuals.

Diversity

Heterozygosity and FIS

Observed and expected heterozygosity per population, plus the inbreeding coefficient FIS. Low heterozygosity populations are flagged against reference baselines for the species.

Plain English

Management Interpretation

Every report section ends with a plain-language summary: what the results mean, what the risks are, and what options exist. Written for program managers and agency staff, not statisticians.

Who it's for

Built for the people
doing the field work

The platform was designed around one constraint: the people with the most valuable wildlife data are often the furthest from the computational tools to use it.

🦌

Wildlife nonprofits

Small organizations running reintroduction programs, camera trap networks, or long-term population monitoring. You have samples. You have sequencing budgets. You need someone to read the results.

"We had four years of wolf genetic data we couldn't interpret."

🏛️

State wildlife agencies

Division of Wildlife Resources offices, fish and game departments, and tribal wildlife programs that need genetic evidence to support translocation decisions, management plans, or ESA petitions.

"Our biologist retired and took the pipeline with her."

🎓

Undergraduate research labs

Conservation biology programs that want to give students real population genetics experience without requiring a semester of bioinformatics prerequisites before they can touch the data.

"Students could finally work with their own field data."

Pricing

Priced for
conservation budgets

Starter
Free

For small orgs evaluating whether this fits your workflow. No credit card needed.

5 analyses per month
Up to 50 samples per VCF
PCA + ADMIXTURE
PDF report download
1 team member
Get started →
Agency
$249 / month

For state agencies and larger programs with multiple concurrent monitoring projects.

Unlimited analyses
Unlimited samples and team members
White-label PDF reports
API access
Priority support
Custom species reference panels
Contact us →

The platform is built on open science

All pipelines running under the hood are open-source and peer-reviewable. We don't hide the analysis behind a black box. The platform is a usability layer on top of methods the conservation genetics community already trusts.

PLINK2 ADMIXTURE Weir-Cockerham FST vcftools Open output format
Our open-science mission →
Get started

Try it on
your own data

The free tier takes a VCF through the full pipeline. No credit card, no installation. If your sequencer can return a file, this platform can read it.

Questions? Email support@rewildgenomics.com — response within 48 hours.